C-termini Analysis of Monoclonal Antibody Fragmentation. J Pharm Res 2016, 1(1): 000102
نویسنده
چکیده
Abbreviations: mAbs: Monoclonal Antibodies; CQA: Critical Quality Attribute; CDRs: Complementary Determining Regions; FC: Fragment Crystallizable; FAB: Fragment Antigen-Binding; SEC: Size-Exclusion Chromatography; SDS-PAGE: Sodium Dodecyl SulfatePolyacrylamide Gel Electrophoresis; CE: Capillary Electrophoresis; IEX: Ion Exchange Chromatography; RPC: Reversed Phase Chromatography; MS: Mass Spectrometry; CNBr: Cyanogen Bromide; ProC-TEL: Protein C-Termini by Enzymatic Labeling; CP Y: Carboxypeptidase Y; SCX: Strong Cation Exchange; COFRADIC: Combined Fractional Diagonal Chromatography; NHS: N-Hydroxysuccinimide; C-TAILS: C-Terminal Amino-Based Isotope Labeling of Substrates; TMT: Tandem Mass Tag; iTRAQ: Isobaric Tag for Elative and Absolute Quantitation
منابع مشابه
Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species. Specifically, glycan structure, sites, relative abundan...
متن کاملAntibody responses to the Bordetella pertussis virulence factor P.69 Pertactin
Bordetella pertussis, the causative agent of whooping cough, is re-emerging in several countries with a traditional high vaccine uptake. Analysis of clinical isolates revealed antigenic divergence between vaccine strains and circulating strains with respect to the B. pertussis virulence factor P.69 Pertactin. Polymorphisms in P. 69 Pertactin are mainly limited to regions comprised of amino acid...
متن کاملProduction of Monoclonal Antibody against Prokaryotically Expressed G1 Protein of Bovine Ephemeral Fever Virus
Epitope-G1 of bovine ephemeral fever virus (BEFV) G glycoprotein has been genetically and antigenically conserved among various isolates of BEFV and only reacts with anti-BEFV neutralising antibodies. Therefore, it is a candidate antigen for development of the enzyme linked immunosorbent assay (ELISA) for serological identification bovine ephemeral fever (BEF)-infected animals. The aim of this ...
متن کاملMonoclonal Antibody Production Against Vimentin by Whole Cell Immunization in a Mouse Model
Background: Pancreatic carcinoma is the fourth-leading cause of cancer death in the United States and due to its late presentation, only few patients would be candidates for the curative treatment of pancreactomy. Monoclonal antibodies have brought hope to targeted therapy.Objectives: To identify new biomarkers, a panel of monoclonal antibodies was genera...
متن کاملCan p53 status resolve paradoxes between human and non-human retinoblastoma models?
brain capillaries and crossreactivity of one antibody with a muscle protein. J Histochem Cytochem 1989;37:159–64. (34) Beaulieu E, Demeule M, Pouliot JF, Averill-Bates DA, Murphy GF, Beliveau R. P-glycoprotein of blood brain barrier: cross-reactivity of Mab C219 with a 190 kDa protein in bovine and rat isolated brain capillaries. Biochim Biophys Acta 1995;1233:27–32. (35) Rao VV, Anthony DC, Pi...
متن کامل